![]() This medium is a simple medium composed of gelatin, peptone and beef extract. The presence of gelatinases is detected using a nutrient gelatin medium. The amino acid is taken up by the cell and used for metabolic purposes. The reaction occurs in two sequential steps: in first reaction gelatinases hydrolyze gelatin into polypeptides and then polypeptides are further converted into amino acids as shown in figure. ![]() This test is used to determine the ability of an organism to produce extracellular proteolytic enzymes, gelatinases that hydrolyze gelatin. Gelatin dissolves in water at 50 degree Celsius and exists as liquid above 25☌ and solidifies or gels when cooled below 25☌. ![]() Robert Koch used nutrient gelatin as an early type of solid growth medium. It has been used as a solidifying agent in food for a long time. Gelatin is a protein derived from the animal protein collagen, a component of connective tissue and tendons in human and other animals.
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